The interaction between EAP30 and ELL is modulated by MCM2

Abstract

ELL-associated protein 30 (EAP30) was initially characterized as a component of the Holo–ELL complex, which contains the elongation factor ELL. Both ELL and Holo–ELL stimulate RNA pol II elongation in vitro. However, ELL and not Holo–ELL inhibits RNA pol II initiation. It is not clear how these two discrete functions of ELL are regulated. Here we report that mini-chromosome maintenance 2 (MCM2) binds to EAP30 and show that MCM2 competes with ELL for binding to EAP30 thus potentially modulating the stability of Holo–ELL. Structured summary MINT- 7277033: EAP30 (uniprotkb: Q96H20) physically interacts (MI: 0915) with RPB1 (uniprotkb: P24928) by anti tag coimmunoprecipitation (MI: 0007) MINT- 7277085: EAP30 (uniprotkb: Q96H20) binds (MI: 0407) to ELL (uniprotkb: P55199) by pull down (MI: 0096) MINT- 7277072: EAP30 (uniprotkb: Q96H20) physically interacts (MI: 0915) with ELL (uniprotkb: P55199) by anti tag coimmunoprecipitation (MI: 0007) MINT- 7277100: EAP30 (uniprotkb: Q96H20) physically interacts (MI: 0915) with ELL (uniprotkb: P55199) by competition binding (MI: 0405) MINT- 7277153: MCM2 (uniprotkb: P49736) binds (MI: 0407) to ELL (uniprotkb: P55199) by pull down (MI: 0096) MINT- 7276989: EAP30 (uniprotkb: Q96H20) physically interacts (MI: 0915) with MCM2 (uniprotkb: P49736) by pull down (MI: 0096) MINT- 7277005: EAP30 (uniprotkb: Q96H20) physically interacts (MI: 0915) with RPB1 (uniprotkb: P24928) by pull down (MI: 0096) MINT- 7276960, MINT- 7277168: MCM2 (uniprotkb: P49736) physically interacts (MI: 0915) with EAP30 (uniprotkb: Q96H20) by two hybrid (MI: 0018) MINT- 7276971, MINT- 7277121, MINT- 7277137: MCM2 (uniprotkb: P49736) binds (MI: 0407) to EAP30 (uniprotkb: Q96H20) by pull down (MI: 0096) MINT- 7277018, MINT- 7277061: EAP30 (uniprotkb: Q96H20) physically interacts (MI: 0915) with MCM2 (uniprotkb: P49736) by anti tag coimmunoprecipitation (MI: 0007)