The Insulin-like Growth Factor-1 (G>A) and 5,10-methylenetetrahydrofolate Reductase (C677T) Gene Variants and the Serum Levels of Insulin-like Growth Factor-1, Insulin, and Homeostasis Model Assessment in Patients with Acne Vulgaris.

Sakineh Zinati-Saeed,Asad Vaisi-Raygani,Mona Akbari,Ziba Rahimi,Fariba Najafi,Mehrali Rahimi,Fariborz Bahrehmand,Ali Ebrahimi,Zohreh Rahimi,Ebrahim Shakiba

doi:10.30699/ijp.2019.105695.2098

Sakineh Zinati-Saeed, Asad Vaisi-Raygani + Show 8 more

Open Access

https://doi.org/10.30699/ijp.2019.105695.2098

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Abstract

Background & Objective:To find an association between gene variants of insulin-like growth factor-1 (IGF-1) and 5,10-methylenetetrahydrofolate reductase (MTHFR) with the risk of acne vulgaris (AV).Methods:In a case-control study, we investigated 150 AV patients and 148 healthy individuals (aged 18-25 years) for the IGF-1 G>A and MTHFR C677T polymorphisms, as well as the serum levels of IGF-1, insulin, and the homeostasis model assessment of insulin resistance (HOMA-IR). The serum biochemical parameters and the genotypes of IGF-1 G>A and MTHFR C677T were detected by using appropriate kits and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods, respectively.Results:The frequencies of IGF-1 and the MTHFR polymorphisms were not significantly different comparing patients and controls. The serum level of IGF-1 was 179.8±72.8 µg/L in AV patients compared to 164.6±63.7 µg/L in controls (P=0.056). The serum level of insulin in female patients was significantly higher than controls. The HOMA was 3.54±5.6 in patients compared to 1.16±1.4 (P<0.001) in controls. Significantly higher levels of fasting blood sugar (FBS), total cholesterol, and low-density lipoprotein-cholesterol (LDL-C) were detected in female patients than controls. However, the level of estradiol was significantly lower in female patients than in controls. In females, the presence of the MTHFR T allele was associated with significantly higher levels of FBS and LDL-C, as well as a significantly lower level of estradiol compared to those carriers of the C allele.Conclusion:We found the absence of an association between IGF-1 and MTHFR polymorphisms with the risk of AV. However, increased insulin, IGF-1, and HOMA levels in AV patients indicated the effect of insulin and insulin resistance in the risk of AV and its severity.

Highlights

Acne vulgaris (AV) is a chronic inflammatory skin disease, which is common during adolescence and in young adults
The presence of the methylenetetrahydrofolate reductase (MTHFR) T allele was associated with significantly higher levels of fasting blood sugar (FBS) and lowdensity lipoprotein-cholesterol (LDL-C), as well as a significantly lower level of estradiol compared to those carriers of the C allele
We found the absence of an association between insulinlike growth factor-1 (IGF-1) and MTHFR polymorphisms with the risk of AV

Summary

Introduction

Acne vulgaris (AV) is a chronic inflammatory skin disease, which is common during adolescence and in young adults. This disease impairs various aspects of life quality [1]. Clinical features of AV include seborrhea (excess grease), inflammation, abnormal follicular keratinization, and various degrees of scarring. The insulin hormone has various roles in body metabolism. The insulin gene is located at the end of the short arm of chromosome 11, synthesized in β cells of the pancreas, and released in response to blood glucose to decrease it [3]. To find an association between gene variants of insulinlike growth factor-1 (IGF-1) and 5,10-methylenetetrahydrofolate reductase (MTHFR) with the risk of acne vulgaris (AV)

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