Abstract
Six restriction fragments of Ad2 DNA which contain sites for RNA initiation have been identified by their ability to hybridize nascent labeled RNA <1 kb in length. Four RNA initiation sites early in infection are identified in regions where previous work ( Flint, 1977) had mapped mRNAs. The major late RNA initiation site is the origin of a giant nuclear transcript extending from ∼16.3 map units to (or close to) 100 units at the end of the genome. This transcription unit encompasses at least four or five mRNA sites; processing of this long transcript appears necessary to generate the mRNA.
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