The inhibitory effects of soy daidzein on obesity in C57BL/6J mice fed high fat

Abstract

The study was performed to examine the effects of soy daidzein on obesity model mice induced by high fat in long period(12 weeks). Four week old male C57/BL6J mice, known as a good model of diet-induced obesity, were fed high fat(36% of total calorie) diets containing various daidzein supplement(0, 100, 500, 1000, 2000ppm) or normal fat (16% of total calorie) diet in terms of confirming obesity induction. High fat-induced increase in body weight, daily weight gain and epididymal fat pad were attenuated by daidzein supplementation. Especially, daily body weight and epididymal fat pad were lower in 1000ppm or 200ppm daidzein supplemented group than normal fat diet group(p<0.05). This fat pad’s decrease was attributed to the decrease of adipocyte number in 1000ppm and 2000ppm daidzein supplemented groups(p<0.05). Serum and liver total lipid increased by high fat were declined dose-dependently by daidzein supplementation(p<0.05). And liver triglyceride was lower in 1000ppm and 2000ppm daidzein supplemented groups than both high and normal fat diet groups(p<0.05). Leptin and adiponectin concentrations when they were adjusted per gram of fat weight were increased but ghrelin was decreased by daidzein supplementation(p<0.05). Fatty oxidation-related genes (5 AMP-activated protein kinase, acyl Coenzyme A oxidase, acyl Coenzyme A sythetase) expression were increased in fold change by 1000ppm daidzein supplementation. These results indicate that soy daidzein can prevent obesity through obesity-involved genes and energy-related hormones regualtion