The Inhibitory Effects of Midazolam and Diazepam on Smooth Muscle Tension in Rat Trachea

Abstract

Background: It has been demonstrated that a group of minor tranquilizers, benzodiazepines, are able to relax airway smooth muscle, but the mechanism by which these agents produce muscle relaxation are not fully understood. This study was undertaken to determine the effects and mechanism of diazepam and midazolam on Ca²+and K+ channel in isolated rat trachea muscles by measuring isometric tension. Methods: Our experiment was performed to evaluate the effects of midazolam and diazepam by cumulative administration from 10-6 M to 3×10-5 M to tracheal smooth muscle contraction which was induced by contractile agonists such as ACh 10-5 M, carbachol 3×10-7 M, and KC1 40 mM. The effects of midazolam and diazepam were evaluated on Ca²+ and K+ channels by inhibition of contraction using a nonspecfic K+ channel blocker such as tetraethyl ammonium (TEA) elicited by a 2 mM Ca²+ space addition to Ca²+ free on high K' depolarizing rat tracheal muscle. Also, to elucidate any mechanism involved, the effects of flumazenil (a specific central antagonist of benzodiazepines), propranolol (a beta adrenergic antagonist), and atropine (a muscarinic antagonist) and tracheal epithelium removal were examined. Results: In a concentration-dependent way, both midazolam and diazepam relaxed airway smooth muscle directly and had inhibitory effects on voltage-dependent Ca²+ (VDCC) and K channels. Conclusions: These results suggest that benzodiazepines relax airway smooth muscle, not via a neural pathway or benzodiazepine receptor but through a direct action on Ca²+ and K+ channels. Benzodiazepine enhanced K+ conductance, leading to a decrease in VDCC opening, thus reducing Ca²+ thtough the voltage-dependent Ca²+ channel, in addition to inhibiting of intracellular Ca²+ release.