Abstract
The inhibitory effect of urea on the alkaline P-nitrophenylphosphatase activities of human tissue extracts is greatly enhanced by the presence of urease. The tissue enzymes may be classified into three groups according to their sensitivities to the urea-urease system. The liver, bone and kidney enzymes are the most sensitive, and the intestinal mucosal enzyme the least affected. The placental enzyme was strongly inhibited by urease in the presence of 0.25–0.5 M urea, but less so at higher concentrations. The action of the urea-urease system appears to be due to the liberation of ammonia since at the pH used (9.3), ammonium salts significantly inhibit the phosphatase activities of all the tissues studied. The intestinal enzyme is the most resistant. Inhibition of alkaline phosphatase with the urea-urease system does not appear to have any diagnostic significance.
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