The inhibitory effect of the leukotriene receptor antagonist on leukotriene D 4-induced MUC2/5 AC gene expression and mucin secretion in human airway epithelial cells

Abstract

Objectives Mucin gene expression and mucin production are markedly increased in inflammatory airway disorders such as asthma, chronic bronchitis and rhinosinusitis. Cytokines, lipopolysaccharides and other inflammatory mediators such as prostaglandin and leukotriene are related to the secretion and production of mucin. However, the relationship of leukotrienes with mucin genes expression is not clear. The aim of this study is to evaluate MUC2/5 AC gene expression and mucin secretion by the leukotriene receptor in human airway epithelial cells. Methods The effect of leukotriene D 4 and the leukotriene receptor antagonist, pranlukast hydrate (ONO-1078) on the regulation of MUC2/5 AC gene expression and mucin secretion were observed in human airway NCI-H292 epithelial cells. The mRNA levels of MUC2/5 AC and the amount of mucin were determined by reverse transcription-polymerase chain reaction (RT-PCR) and immunoassay. Results Leukotriene D 4 upregulated MUC2/5 AC gene expression and mucin secretion in a dose dependent pattern. Pranlukast hydrate (ONO-1078, 100 μM) downregulated the leukotriene D 4-induced MUC2/5 AC gene expression and mucin secretion. Conclusion These results suggest that the leukotriene receptor system is one of the mechanisms related to MUC2/5 AC gene expression and mucin secretion in the human airway epithelium.