The inhibitory effect of sinigrin on the production of inflammatory mediators induced by lipopolysaccharide in RAW 264.7 macrophages (1056.5)

Abstract

Sinigrin (2‐propenylglucosinolate) is a biochemical nutrient found naturally in some plants of the Brassicaceae family such as broccoli, Brussels sprouts. Recent studies showed that sinigrin affect the preventing of cancer in preclinical or clinical trials for cancer chemoprevention. However, anti‐inflammatory effect of sinigrin was unknown. In the present study, we examined the effects of sinigrin on the production of inflammatory mediators in lipopolysaccharide (LPS)‐activated RAW 264.7 macrophages. The cell viability was determined by MTT assay. We investigated the anti‐inflammatory effects of sinigrin on Nitrite Oxide (NO) and pro‐inflammatory cytokine production by colorimetric assay and ELISA assay, respectively. We also examined the expression of MAPK, NLRP‐3 and p65 by Western blot. The results showed that NO production was not reduced by sinigirn.Whereas, sinigrin inhibited the levels of tumor necrosis factor‐ α (TNF‐α) and interleukin‐6 (IL‐6). Sinigrin blocked phosphorylation of JNK and p38, but not that of ERK. In addition, sinigrin treatment significantly suppressed the expression of p65 and NLRP‐3 compared with the control. These finding suggest that sinigrin has an anti‐inflammatory effects, which may result from inhibition of MAPK phosphorylation, and the expression of NLRP‐3 and p65, thereby decreasing the production of pro‐inflammatory mediators.