The Inhibitory Effect of Curcumin on Ornithine Decarboxylase against Hepatic Carcinoma

Abeer A Khamis,Amal H Mahmoud,Tarek M Mohamed,Amira H Sharshar

doi:10.4236/jbm.2019.75015

Abeer A Khamis, Amal H Mahmoud + Show 2 more

Open Access

https://doi.org/10.4236/jbm.2019.75015

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Abstract

Curcumin the active component of turmeric is widely used as an anticancer agent for treating many human cancers. This study aimed at the extraction of curcumin from Curcuma Longa and investigates its therapeutic effect as ornithine decarboxylase (ODC) inhibitor in HepG2 cells. The proliferation of HepG2 cells was carried out by using the MTT assay. In addition, cell cycle analysis was evaluated by using the flow-cytometric technique. Our results showed that curcumin has the ability to inhibit the proliferation of HepG2 cells with IC50 of 24.79 μg/ml and induced G2/M cell cycle arrest. Moreover, it caused an elevation in the intracellular concentration of Ca2+. Moreover, in the curcumin administration the downregulation expression level of ODC and Bcl-2 genes (p ≤ 0.05) was significant found. On the other hand, upregulation in the expression level of P53, Bax, and caspase-3 genes (p ≤ 0.05). This study concluded that curcumin may be considered as a new saving candidate for the future progress of antitumor agents.

Highlights

Cancer is considered as a complex genetic disease that is caused primarily by environmental factors
Our results showed that curcumin has the ability to inhibit the proliferation of HepG2 cells with IC50 of 24.79 μg/ml and induced G2/M cell cycle arrest
Our finding indicated that treatment with curcumin-induced apoptosis of the tumor cells by the upregulation in the expression level of the P53 gene in HepG2 cells after administration of curcumin in a time-dependent manner

Summary

Introduction

Cancer is considered as a complex genetic disease that is caused primarily by environmental factors. The cancer-causing agents can be present in food and water, in the air, and in chemicals and sunlight that people are exposed to [1]. Primary liver cancer is considered the second most common cancer leading to death and sixth most diagnosed cancer worldwide [2]. Hepatocellular carcinoma is the vast malignant tumor it accounts for 85% - 90% of primary liver cancers.

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