Abstract
The spread of antibiotic-resistant pathogens has resulted in the need for new treatments. The aim of the present study is to investigate the effect of bacteriocin from Lactobacillus acidophilus ATCC 4356 and Lactobacillus plantarum ATCC 8014 on planktonic and biofilm forms of Serratia marcescens strains. The direct antagonism of the L. plantarum and L. acidophilus cell-free supernatant on S. marcescens cultures was determined using an optical density assay. The bacteriocin was partial purified by ammonium sulfate precipitation. Its molecular weight was analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The effect of bacteriocins on the biofilm of S. marcescens strains was then determined with 2,3,5-triphenyl tetrazolium chloride. The purified bacteriocin from L. plantarum ATCC 8014 and partially purified bacteriocin from L. acidophilus ATCC 4356 displayed noticeable inhibitory activity against planktonic and biofilm forms of S. marcescens strains. SDS-PAGE analysis revealed that the apparent molecular weight of bacteriocin from L. planetarium was 63 kDa, and that of bacteriocin from L. acidophilus was 68 or 48 kDa. The bacteriocins could be effective compounds to control surface-attached pathogenic bacteria and can be used as therapeutic agents after acceptable in vivo experimentation.
Highlights
Biofilm is a structured community of bacterial cells enclosed in a self-produced polymeric matrix that adheres to biotic and abiotic surfaces [1]
The biofilm structure increases the survivability of bacteria by 1000 times when they are exposed to high concentrations of antimicrobial agents, such as antibiotics, when compared with the planktonic form [2]
The inhibitory effect of L. plantarum and L. acidophilus supernatant measured by microscale optical density assay (MODA) The inhibitory effect of Cell-free supernatant (CFS) of Lactobacillus plantarum and L. acidophilus on S. marcescens could be attributed to the presence of bacteriocin-like substance (BLS)
Summary
Biofilm is a structured community of bacterial cells enclosed in a self-produced polymeric matrix that adheres to biotic and abiotic surfaces [1]. The limitation of the penetration of antimicrobial compounds into the biofilm structure is one of the most important reasons for the high resistance of bacteria in biofilm [3,4]. This characteristic is medically important because it results in the contamination of hospital equipment and medical implants [5,6]. According to a report by the National Institutes of Health, nearly 70% of microbial infections are associated with a bacterial biofilm [5]. The killing and removal of biofilm cells are the main targets of infection control strategies [7]
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