Abstract
The effect of oil on the number of local lesions produced by tobacco mosaic virus (TMV) in Nicotiana glutinosa L. leaves was studied. Application of oil before or after inoculation of the leaves affected the number of local lesions produced to the same extent. An equivalent inhibition was found when nucleic acid of TMV was used, indicating that uncoating of the virus is not sensitive to or influenced by oil. Oil treatment at different times after inoculation showed that the lowest number of local lesions was produced by the leaves with oil at 15 min after inoculation. The results suggest that not every infecting particle is sensitive to oil at the same moment. Repeated treatments at intervals of 15 min over a period of 2 hr caused an almost complete inhibition.
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