Abstract
<p>Abstract<br />Contamination of pathogen bacteries at the fresh fish may occur during the post harvesting to the consuming period, and endanger human health. One of simple and safe way to protect secureness of fresh fish food is the use of Caulerpa lentillifera to push down pathogen bacteries activity. The aims of this research to investigate lability of sea grape (Caulerpa lentillifera) against the activity of pathogen bacteries Escherichia coli, Vibrio cholerae and Salmonella sp., in fresh fish, during storage phase, at ambient and ice temperatures. Method used in this research is experimental laboratories method, that is, 10%, 20% and 30% blended sea grapes applied on fresh fishes, Selar crumenopthalmus during storage of 1, 2 and 3 days at ambient and ice temperatures. All the applied of blended of Caulerpa lentillifera, shows the ability to obstruct the activity of bactery group coliform and Escherichia coli on fresh fish Selar crumenopthalmus up to 2x 24 hours, at ambient temperature, and 3x24 hours at ice temperature. During storage period, the occurance and grows of Salmonella sp. and Vibrio cholerae bacteries is undetected. Fresh sea grapes concentration of 10% is sufficient to be applied on fresh fish to obstruct the activity of pathogen bacteries Escherichia coli during storage time of 2 x 24 hours at room temperature, and 3 x 24 hours at ice temperature, and to block the occurance of Vibrio cholerae and Salmonella sp. during storage period.</p>
Highlights
Contamination of pathogen bacteries at the fresh fish may occur during the post harvesting to the consuming period, and endanger human health
One of simple and safe way to protect secureness of fresh fish food is the use of Caulerpa lentillifera to push down pathogen bacteries activity
Method used in this research is experimental laboratories method, that is, 10%, 20% and 30% blended sea grapes applied on fresh fishes, Selar crumenopthalmus during storage of 1, 2 and 3 days at ambient and ice temperatures
Summary
Bahan yang digunakan dalam penelitian ini adalah ikan Selar crumenopthalmus segar yang dibeli dari nelayan desa Hative Besar Kota Ambon, Caulerpa lentillifera segar dari perairan Desa Letman Kabupaten Maluku Tenggara, es curai dan media untuk analisis bakteri patogen yaitu Buterffield Phosphate Buffered (Merc), Plate Count Agar (Difco), Laktosa Broth (Merc), Tetrathionate Base Broth (Difco), Hektoen Enterik (Difco), Bismuth Sulfate Agar (Difco), Xylose Lysine Desoxycholate (Difco), Triple Sugar Iron Agar (Difco), Lysine Iron Agar (Difco), Urea Broth (Difco), Simmon Citrate Agar (Difco), Lactose (Difco), Tryptose Broth (Difco), M-R Up (Difco), Lauryl Tryptose Broth (Difco), Levine’s Eosin Methylene Blue Agar (BDBBL), Brillian Green Lactose Bile Broth (Difco), MR-VP Medium (Difco), EC Medium (Difco), Brillian Green Lactose Blue Agar (Difco), TCBA(Difco), TSA (Difco), NaCl (K 45005604342), KIA (BDBBL). Alat-alat yang digunakan yaitu timbangan Ohause (ARB 120), thermocouple (tipe 52 II), stomacher (serial no.050716015), autoclave (HL 36 A), inkubator (IMB 500), oven (UM 400), lemari pendingin (G55-388), vortex (F 202A0175), cawan petri (pyrex), erlenmeyer (pyrex), pipet (A EX 20OC), pipet (boy accu jet 01J16302)
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