The Inhibitive Effect of Salidroside on Apoptosis of EA.hy926 Cells Induced by H2O2 under Simulated Microgravity

Abstract

Microgravity or simulated microgravity(SM) can induce cardiovascular dysfunction in which vascular endothelial cells play an important role. Studies showed that oxidative stress was common in vascular tissues under simulated microgravity and was also increased in vascular endothelial cells during spaceflight. The purpose of this experiment is to study whether vascular endothelial cells have different biological response to oxidative stress under simulated microgravity with new generation transcriptome sequencing technology and discuss the cell protection effect of salidroside. EA.hy926 cells, a fusion of HUVECs and the lung carcinoma cell line A549, were randomly divided into control group(Con72) and clinostat group(Z72), and after clinostating for 72 hours, the two groups were randomly given the following treatment at the same time: stimulated by H2O2, stimulation by H2O2 and salidroside intervention. Apoptosis was measured by flow cytometry and transcriptome sequencing was performed by new generation sequencing technology, RT‐qPCR was used to verify the differentially expressed genes and detect the effect of salidroside on the expression of these genes. The results showed that there were obvious apoptosis in EA.hy926 cells treated by H2O2 under simulated microgravity, which was significantly inhibited by salidroside. There were six significant differentially expressed genes screened by transcriptome sequencing, including BCL‐2A1, FAM196B, TMEM158, PPP1R16B, CXCL8, PPP1R3B. RT‐qPCR results showed that BCL‐2A1 and FAM196B were consistent with the transciptome sequencing results, meanwhile, BCL2A1 and FAM196B genes were significantly increased after salidroside intervention in H2O2‐treated EA.hy926 cells under simulated microgravity. These results showed that apoptosis was induced significantly in H2O2‐treated EA.hy926 cells under simulated microgravity, which can be inhibited by salidroside, the active ingredient of Rhodiola rosea, through the regulation of BCL‐2A1 and FAM196B gene expression.Support or Funding InformationFunded by State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, grant No.SMFA14B01This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.