Abstract
Summary By comparing the amount of inhibition of precipitation produced by human serum in a rabbit antihuman globulin-labeled γ-globulin system with the amount of inhibition produced in the same system by a known amount of purified γ-globulin, human serum levels of γ-globulin can be determined precisely. When this method was applied to eluates obtained from conventional starch fractionation, only those proteins which migrated electrophoretically as γ-globulins exerted a significant inhibitory effect.
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