The inhibition effects of Camellia japonica leaf extract on antigen-stimulated degranulation and inflammatory cytokines in mast cells

Abstract

This study investigated the effects of leaf extract of Camellia japonica (LECJ) on antigen-stimulated degranulation in mast cells and its mechanism of action in cell lines. It was identified that the inhibitor activation in the examination of β-hexosaminidase, inflammatory cytokines and Syk kinase activation upon which LECJ were collected in the prime of antioxidant activity were 1/10 concentration lower than the patent published in 2007 and the paper published 2008. In the case of preparing LECJ, the inhibition of the concentration was 10 times greater than the start as reported in the existing paper published in 2007 and the patent published 2008. The effects of LECJ verified it as an effective material for cosmetic use. In the study, the antigen-stimulated activation of mast cells, the inhibition of degranulation on LECJ and its mechanism of action, LECJ exhibited an inhibitory effect on degranulation in mast cells. Also, the activation of signaling pathways in antigen-stimulated mast cells depends initially on the interaction of FceRI on the downstream activation of Syk and other tyrosine kinases. This study showed that degranulation in antigen-stimulated mast cells and the secretion of inflammatory cytokines decreased in a concentration dependent manner. These results showed that the LECJ in cell line is effective for its anti-allergic activity. In the case of LECJ, the study showed that the effects of degranulation, the effects of the expression and secretion of inflammatory cytokines, and the directly inhibiting activation of signaling molecules such as adaptors.