The inhibition by human MSCs-derived miRNA-124a overexpression exosomes in the proliferation and migration of rheumatoid arthritis-related fibroblast-like synoviocyte cell

Hong-Yan Meng,Li-Hui Chen,Li-Qing Chen

doi:10.1186/s12891-020-3159-y

Abstract

BackgroundRheumatoid arthritis is a long-term, progressive autoimmune disease. It is characterized by synovial hyperplasia leading to swelling, stiffness, and joint deformity in more than one joint. Fibroblast-like synoviocytes are the major cell types that make up the synovial intima structure, which is one of the decisive factors in the development and course of rheumatoid arthritis.MethodsThe potential therapeutic effects of MSCs-derived miRNA-124a overexpression exosomes were evaluated in vitro by the method including MTT assay and cell cycle test for cell proliferation, scratch wound closure and transwell for cell migration, flow cytometry and western for the apoptosis detection.ResultsExosomes derived from human MSCs that overexpression miRNA-124a were prepared and characterized. We found that the pretreatment of this exosome was able to inhibit the proliferation and migration of fibroblast-like synoviocyte cell line and promote the apoptosis of this cell during the co-incubation.ConclusionsExosomes derived from MSCs were proved to be a suitable vector for the delivery of therapeutic miRNA-124a, and such miRNA-124a overexpression exosomes were expected to provide a new medicine and strategy for the treatment of rheumatoid arthritis.

Highlights

Rheumatoid arthritis is a long-term, progressive autoimmune disease
CD9 and CD63 that measured by Western Blot and flow cytometry was specific surface markers of exosomes
We found that human MSCs (HMSCs)-124a-EV significantly inhibits the proliferation of MH7A cells compared with Human mesenchymal stem cells derived exosomes (HMSC-EV)

Summary

Introduction

Rheumatoid arthritis is a long-term, progressive autoimmune disease. It is characterized by synovial hyperplasia leading to swelling, stiffness, and joint deformity in more than one joint. Fibroblast-like synoviocytes are the major cell types that make up the synovial intima structure, which is one of the decisive factors in the development and course of rheumatoid arthritis. Fibroblast-like synoviocytes (FLS) are the major miRNAs regulate many physiological processes at the transcriptional and post-transcriptional level [6]. Increasing evidence has revealed that altered expression of miRNA in FLS and synovial tissue is closely related to the development of RA [10]. Takuya Niimoto et al reported that miR-146a was closely related to IL-17 regulation in the FLS in RA patients [11]. All the above mentioned suggest that miRNA plays an important role in the development of RA, which may become a potential therapeutic target in the treatment of RA

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Conclusion