The influences of graphene oxide and nitrification inhibitor on vegetable growths and soil and endophytic bacterial communities: Double-edge sword effects and nitrate risk controls

Abstract

Crop yield and quality are substantial indicators of evaluating agricultural nitrogen management practices, and the nitrate (NO3−-N) is one of the predominant factors affecting crop quality. The NO3−-N accumulation in vegetable crop affects plant growth and quality and human health. Therefore, it is necessary to stimulate vegetable yield but eliminate excessive NO3−-N in soils and plants with feasible management strategies. Graphene oxide (GO) is a novel carbon nanomaterial that has attracted great attention, but rare research has been conducted to quantify the effects of GO on plant NO3−-N accumulation and microbial communities. This study explored effects of the GO and nitrification inhibitors, dicyandiamide (DCD) and 3, 4-dimethylpyrazole phosphate (DMPP), on vegetable yields and NO3−-N contents and bacterial communities in soil-cabbage (Brassica rapa subsp. Chinensis) system. The soil NO3−-N content was significantly reduced with the single GO application. The cabbage NO3−-N content was increased by 60.4 % while the cabbage yield was significantly enhanced by 101.9 % with the single GO application. Meanwhile, the Invsimpson index of soil bacterial community and the ACE and Chao1 richness estimators of endophytic bacterial community were significantly decreased by the GO application. Cabbage NO3−-N content was significantly and negatively correlated with the soil Myxococcota, endophytic bacterial community co-occurrence network edge, cabbage soluble sugar and cabbage proline. The GO application generated double-edged sword effects of positively promoting yield but causing risks of NO3−-N accumulation and quality deterioration. However, these adverse effects could be mitigated by the extra nitrification inhibitor application. The potential ecological risks of GO application to the vegetable quality and endophytic community should be considered.