The influence of vitamin‐mineral supplementation with prolonged action on cows and on the protein metabolism in calves

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It is well established that one of the pathology‘ reason in calves is the disturbances of prenatal development as a result of vitamin and mineral abstinence. The aim of the study was to research the influence of vitamin‐mineral bolus’ supplementation with prolong action to the ration of cows on the calves’ protein metabolism. The studies were carried out in one of the breeding farms of the Leningrad region. Holstein cows were used aged from 3 to 5 years and their calves. 5 groups were formed (n=5 by each). The cows of the 1st experimental group have received two boluses All – mineral plus (the compound: Mg, Zn, Cu, Co, Se, I, Vitamin A, D3, E, Holland Animal Care, Holland), 2d experimental – two boluses Uno Biotin (the compound: Mg, Zn, Cu, Co, Se, I, Vitamin A, D3, E, Holland Animal Care, Holland), 3d experimental group ‐ one Cattle bolus with Iodine (the compound: Cu, Co, Se, I, Telsol Limited, United Kingdom), 4th experimental group – one Cattle Bullet (the compound: Mg, Zn, Cu, Co, Se, I, Vitamin A, D3, E, Holland Animal Care, Holland). The 5th group was control with ordinary ration vitamin‐mineral diet. All cows of experimental groups have got boluses one time which was 60 days before calving. The prolongation of all boluses was 120 days after receiving except bolus All‐mineral plus. The prolongation of their was 180 days. The compound of these boluses includes manganese, zinc, copper, selenium, cobalt, iodine, Vitamin A, D3, E, H. We used the clinical examination of calves and biochemical analysis of calves blood samples on the 3d‐5th days after calving. Serum was analyzed for total protein, albumins and globulins. We were used Student's t‐tests to study differences between groups. Thus, the clinically health animals were nineteen calves of experimental groups and second calves if control group. The body mass of newborn calves was 35‐40 kg. The calves were stood up during first two hours after calving and had a healthy sucking reflex. The temperature of their was fluctuated within 38.5‐39.30°С. The results of biochemical analysis showed that the level of total protein was within the physiological norm in the bloods’samples of 1st, 3d, 4th and 5 th calves groups. The level of total protein was statistically increased in the calves of 1st group in 1.4 times (р<0,05) compared with data of calves in the control group. The value of albumins of calves was decreased in 1.07 times (р<0,05) in 2d group and in 1.4 times in control group (18,19±3,37g/l). We were observed the insignificant increasing trend of albumins’level in calves of 1st,2d,3d and 4th groups compared with data of control group. The difference of globulins’level between calves of 1st, 4th experimental and control group was 1.3 times (р<0,05). The value of globulin in the calves of 2d group was 35,64±5,93 g/l and 3d group ‐ 34,45±7,93 g/l. So, we can assume that the vitamin‐mineral supplementation of cow‐mothers during dry time has a positive influence on the synthesis of the total protein and its fraction ratio.