THE INFLUENCE OF VANADIUM AND CHROMIUM CITRATES ON THE ANTIOXIDANT SYSTEM IN THE BLOOD OF RATS WITH EXPERIMENTAL DIABETES

Abstract

Introduction. Today, diabetes mellitus is one of the most common metabolic diseases. Therefore, there is a growing interest in finding new pharmacological agents for prevention and treatment of diabetes. Aim. To determine the influence of vanadium and chromium citrates on the state of the antioxidant system in the rats’ blood with experimentally induced diabetes. Method s . We determined the content of peroxide oxidation products of lipids in the blood — lipid hydroperoxides and thiobarbituric acid reactive substances; activity of enzymes of antioxidant protection — catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase and the content of reduced glutathione. Results. Hyperglycemia is observed in the blood of the rats with alloxan diabetes and lipid peroxide oxidation — lipid hydroperoxides and thiobarbituric acid reactive substances increased. In addition, for experimental diabetes in the blood of animals, the activity of catalase, superoxide dismutase, glutathione reductase and the content of reduced glutathione decreased, but the content of glutathione peroxidas increased. The content of products of lipid peroxidation and the activity of antioxidant enzymes became normal after the introduction of vanadium and chromium citrates into the diet of the animals with diabetes mellitus. Conclusion. Introduction of citrates of vanadium and chromium into the diet of animals with alloxan-induced diabetes causes the normalization of the state of the antioxidant system in the blood. Obtained results can form the basis for the development of methods and tools for preventing diabetes mellitus.