Abstract
Abstract The retention behaviour of eight proteins in high performance hydrophobic interaction chromatography (HPHIC) in a mobile phase consisting of ammonium sulphate-potassium dihydrogen phosphate containing urea was studied. By using the stoichiometric displacemant model for retention (SDM-R) of solute in liquid chromatography, and by measuring surface tention of the mobile phase in the presence of urea, also and UV-spectra of proteins, it was found that in the presence of urea in the mobile phase of HIC, even though the urea somewhat strong solvent in terms of result, the change of the conformation of protein molecular in hydrophobic interaction systems due to the presence of urea is considered as the major contribution to the retention of proteins.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
