The influence of ultrasonic radiation of low intensity in cultured fibroblast cells

Abstract

Introduction: In vitro and in vivo put in evidence that the Low Intensity Pulsed Ultrasound therapy exerts a significant influence on cellfunction (cytoskeleton organization, stimulation of mitochondrial activity, ATP levels and plasma membrane). Objective: This studywill analyze the radiation of low intensity pulsed ultrasound in fibroblast cells L 929. Method: In this study are presented the data fromeach exposure group average and standard deviation in each moment of evaluation (24 hours, 48 hours and 72 hours). The controlgroup (received no radiation), 0.2 W/cm2 with 10% pulse regime (1: 9 duty cycle), 0.2 W / cm2 with 20% pulse regime (2: 8 cycle work),0.4 W/cm2 with pulse scheme 10% (1: 9 duty cycle), 0.4 W/cm2 with pulse scheme 20% (2: 8 duty cycle). The analyzes will be performedthrough optical microscopy, MTT method 3 - (4,5-dimethylthiazol-2-yl) -2,5 diphenyl tetrazolium bromide, within the incubation timesof 24, 48 and 72 hours. Results: Given the above study, the results presented in this project will be directed to increase the stimulationprocess and proliferation of fibroblast cells from the pulsed ultrasonic irradiation of low intensity, correlating with the healing process,neovascularization and repair. Conclusion: Therefore, the study of the effect of ultrasound from cell culture provides us with a simpleand informative model on the significant aspects of the use of physical therapy in vivo system.