The influence of topical application of grapeseed extract gel on enamel surface hardness after demineralization

Abstract

The aim of this study was to analyze the influence of topical application of 6.5% and 12.5% grapeseed extract gels for duration of application 16 and 32 minutes on the enamel surface hardness following tooth demineralization by an energy drink. The samples were 21 bovine teeth that underwent demineralization by immersion in the energy drink for 5 minutes in an incubator at 37°C. The demineralized specimens were randomly divided into a control group and 2 treatment groups. The control group was immersed in artificial saliva for 6 hours at 37°C, whereas the treatment groups were treated with topical 6.5% and 12.5% grapeseed extract gels for durations of 16 and 32 minutes and then immersed in artificial saliva for 6 hours at 37°C. The hardness was measured with a Knoop hardness tester. Statistical analysis by repeated ANOVA and one-way ANOVA revealed a significant increase in the enamel hardness value (p<0.05) after the application of the topical grapeseed extract gels at both concentrations. Application of 12.5% topical grapeseed extract gel for 32 minutes resulted in a restored hardness that insignificant diffrence from the initial hardness value obtained before demineralization (p>0.05).