The influence of tide-brought nutrients on microbial carbon metabolic profiles of mangrove sediments

Abstract

Mangrove ecosystems in the intertidal zone are continually affected by tidal inundation, but the impact of tidal-driven nutrient inputs upon bacterial communities and carbon metabolic features in mangrove surface sediments remains underexplored, and the differences in such impacts across backgrounds are not known. Here, two mangrove habitats with contrasting nutrient backgrounds in Shenzhen Bay and Daya Bay in Shenzhen City, China, respectively, were studied to investigate the effects of varying tidal nutrient inputs (especially dissolved inorganic nitrogen and PO43−-P) on bacterial community composition and functioning in sediment via field sampling, 16S rDNA amplicon sequencing, and the quantitative potential of microbial element cycling. Results showed that tidal input increased Shenzhen Bay mangrove's eutrophication level whereas it maintained the Daya Bay mangrove's relatively oligotrophic status. Dissolved inorganic nitrogen and PO43−-P levels in Shenzhen Bay were respectively 12.6–39.6 and 7.3–29.1 times higher than those in Daya Bay (p < 0.05). In terms of microbial features, Desulfobacteraceae was the dominant family in Shenzhen Bay, while the Anaerolineaceae family dominated in Daya Bay. Co-occurrence network analysis revealed more interconnected and complex microbial networks in Shenzhen Bay. The quantitative gene-chip analysis uncovered more carbon-related functional genes (including carbon degradation and fixation) enriched in Shenzhen Bay's sediment microbial communities than Daya Bay's. Partial least squares path modeling indicated that tidal behavior directly affected mangrove sediments' physicochemical characteristics, with cascading effects shaping microbial diversity and C-cycling function. Altogether, these findings demonstrate that how tides influence the microbial carbon cycle in mangrove sediments is co-correlated with the concentration of nutrient inputs and background status of sediment. This work offers an insightful lens for better understanding bacterial community structure and carbon metabolic features in mangrove sediments under their tidal influences. It provides a theoretical basis to better evaluate and protect mangroves in the context of global change.