The influence of the transfection of SDF-1α to the heart mediated by recombinant adenovirus, microbubbles and ultrasound on non targeted tissues of rats with acute myocardial infarction

Abstract

Objective To evaluate gene transfection in liver, lung and kidney by ultrasound, microbubble and recombinant adenovirus mediated exogenous stromal cell derived factor-1α (SDF-1α) gene transfer to the heart in rats with acute myocardial infarction(AMI). Methods Forty AMI SD rats were randomly divided into control and experimental groups: myocardial infarction+ ultrasound irradiation group (M+ U/control group, n=10), and 3 experimental subgroups on the basis of pAd-EGFP/SDF-1α (The biotin recombinant adenovirus expressing enhanced green fluorescent protein and SDF-1α). Genes transfection length of time: 1 day, 2 days and 3 days of transfection (M+ S1+ U, M+ S2+ U and M+ S3+ U group). The expression of EGFP in liver, lung and kidney were detected by laser scanning confocal microscopy at 7 days after administration. Results There was a little expression of EGFP in the liver, lung and kidney in the drug delivery group and no expression in the control group.The differences in the expression of EGFP between all the gene delivery groups and the control group were statistically significant(P<0.05). With the increase of the number of medication days, the target gene transfection increased slightly, but there was no significant difference among the different drug delivery groups. Conclusions When the ultrasound, microbubble and recombinant adenovirus mediated exogenous SDF-1α gene transfer to the heart in AMI rats, liver, lung and kidney tissues will also be transfected. However, with the increasing of the days of administration, the transfection of target gene in non-target tissue produces only a slight accumulation. The transfection area of target gene in non-target tissue is not linear correlated with the days of administration. Key words: Ultrasound targeted microbubble destruction; Acute myocardial infarction; SDF-1α; Gene transfection in non-target tissue