Abstract
Background Amnion grafts can be preserved as freeze-dried amnion membrane (FD-AM) and amnion sponge. Preserved grafts require to be sterilized by gamma irradiation. However, each step of the process could affect its biological properties. Even so, there are only a few studies that report the influence of the preservation method and gamma irradiation on growth factor levels in preserved amniotic grafts. Methods This was an in vitro experimental study with a pretest-posttest group design using a consecutive sampling technique in one batch of amnion donors at a particular time. The amnion was made into FD-AM and amnion sponge preparations, and they were sterilized with gamma irradiation (15 kGy and 25 kGy). Nonirradiated specimens served as controls, and 20 mg of each specimen was pulverized to evaluate the growth factors levels using ELISA. Results There were significant decreases in amnion sponge compared to the FD-AM, both in transforming growth factor beta (TGF-β) and basic fibroblast growth factor (bFGF) levels and in the preirradiated and 25 kGy postirradiated preparations (p ≤ 0.05). The growth factor levels in the preirradiated and postirradiated FD-AM (both 15 kGy and 25 kGy) showed significant differences (p ≤ 0.05). Likewise, the preirradiated amnion sponge group's growth factor levels compared with the postirradiated amnion sponge group also showed a significant decrease (p ≤ 0.05). Conclusion TGF-β and bFGF levels were lower in amnion sponge than FD-AM. The FD-AM and amnion sponge preparations' growth factors levels were reduced following gamma irradiation sterilization. Although the decrease in growth factor levels is significant, the number of growth factor levels is still sufficient for tissue healing.
Highlights
Amnion grafts can be preserved as freeze-dried amnion membrane (FD-AM) and amnion sponge
Our study evaluates the influence of both the gamma-ray irradiation and the method of preservation on the TGF-β and basic fibroblast growth factor (bFGF) levels in amniotic grafts, namely, freeze-dried amnion membrane (FD-AM) and the amnion sponge. e FD-AM and amnion sponge preparations can be seen in Figures 1 and 2 below
Is study found that the TGF-β and bFGF levels were lower in the amnion sponge than in the FD-AM. e lessening of growth factors in preserved amniotic is doubtlessly inevitable
Summary
Amnion grafts can be preserved as freeze-dried amnion membrane (FD-AM) and amnion sponge. There are only a few studies that report the influence of the preservation method and gamma irradiation on growth factor levels in preserved amniotic grafts. E amnion was made into FD-AM and amnion sponge preparations, and they were sterilized with gamma irradiation (15 kGy and 25 kGy). Ere were significant decreases in amnion sponge compared to the FD-AM, both in transforming growth factor beta (TGF-β) and basic fibroblast growth factor (bFGF) levels and in the preirradiated and 25 kGy postirradiated preparations (p ≤ 0.05). The preirradiated amnion sponge group’s growth factor levels compared with the postirradiated amnion sponge group showed a significant decrease (p ≤ 0.05). E FD-AM and amnion sponge preparations’ growth factors levels were reduced following gamma irradiation sterilization. The decrease in growth factor levels is significant, the number of growth factor levels is still sufficient for tissue healing
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