The Influence of the Levels and Addition Methods of Zinc on Stimulating some Enzymes to Resist Salinity

Abstract

A field experiment was conducted in Haditha city – Anbar governorate in winter season of 2021-2022, in a loamy texture soil to study the influence of levels and addition methods of Zinc to stimulate some enzymes for increasing the tolerance of wheat crop to salinity. The experiment was designed with randomized complete blocky design with split plots (R. C. B. D), by three replicates included included the experiment included soaking the seeds before planting with zinc in three levels of zinc, the first level (0) which represents the comparison treatment (soaking with water only) and the second level (75) which represents the soaking of the seeds with zinc at a concentration (75 mg Zn kg−1) while the third level included the soaking of the seeds with zinc With a concentration of (150 mg Zn kg−1) for eight hours, then the plant was sprayed with the same concentrations above in two batches, the first at the branching stage and the second was at the plant booting stage.The statistical analysis results showed that Zn addition (seed soaking and foliar spraying), led to reduction of harmful effects of saline stress, as well as increasing Zn concentration in soil, wheat straw, roots, 1000 grain weight m, grains yield and activity of antioxidants enzymes (POD and CAT). The best results have achieved by using the level 150 mg kg−1 Zn with seed soaking and with foliar Zn spraying on plant together and that reduce the effects of saline stress in plant.