Abstract
PURPOSE During physical exertion, fibrinolysis increases to protect against exertion-related ischemic events. Increased fibrinolysis may be partially due to a decrease in plasminogen activator inhibitor-1 (PAI-1), the main circulating inhibitor of tissue plasminogen activator. The 4G/5G polymorphism of the PAI-1 gene is associated with resting PAI-1 plasma concentrations. The present study sought to investigate the influence of the 4G/5G polymorphism of the PAI-1 gene on the exercise-induced PAI-1 response. METHODS Thirty-five healthy, untrained males (mean ± SD age = 26 ± 5yrs, ht = 180.9 ± 8.1 cm, wt = 86.3 ± 14.6 kg) performed a maximal exercise test on a motorized treadmill. Blood samples were obtained via clean venipuncture prior to and immediately following exercise. Blood was drawn into an acidified citrate solution and centrifuged to obtain platelet-poor plasma. PAI-1 activity and antigen were assessed using enzyme-linked immunoadsorbant assay (ELISA). DNA was extracted from whole blood and amplified by polymerase chain reaction (PCR) using allele-specific primers. PCR products were then electrophoresed in a 2% agarose gel and imaged with an ultra-violet transilluminator for genotype determination. The effects of genotype and exercise on PAI-1 were assessed using a repeated measures analysis of variance. RESULTS Resting PAI-1 antigen (4G/4G = 25.74 ± 16.4 ng/ml, 4G/5G = 21.10 ± 14.8 ng/ml, 5G/5G = 17.65 ± 9.0 ng/ml) and resting PAI-1 activity (4G/4G = 18.43 ± 19.7 IU/ml, 4G/5G = 12.41 ± 12.5 IU/ml, 5G/5G = 14.50 ± 17.4 IU/ml) were not different between groups (p > 0.05). There were also no significant differences between groups for post-exercise PAI-1 activity (4G/4G = 11.42 ± 12.9 IU/ml, 4G/5G = 7.94 ± 8.3 IU/ml, 5G/5G = 8.49 ± 10.6 IU/ml) and post-exercise PAI-1 antigen (4G/4G = 24.46 ± 16.0 ng/ml, 4G/5G = 17.87 ± 13.3 ng/ml, 5G/5G = 16.09 ± 9.7 ng/ml). CONCLUSION The 4G/5G polymorphism of the PAI-1 gene does not influence the reduction in plasma PAI-1 observed during exercise.
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