Abstract
The o-acetylserine (thiol) lyase (OAS-TL) gene is part of the plant sulfate assimilation pathway. The synthesis of cysteine, the first product of the pathway containing organic sulfur, has been previously observed to be dependent on the availability of sulfur and activity of OAS-TL. In this study, the ability of Vicia sativa L. to alter its metabolism to compensate for deficiencies brought about by sulfur stress was utilized to elucidate the functionality of Voas-tl5 (GenBank Accession No. DQ456491), a gene cloned from Vicia sativa. The transcription levels of Voas-tl5 increased in response to sulfur deficiency, indicating that the gene was active in the cysteine biosynthetic pathway.
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