Abstract

Growth and survival of alfalfa rhizobia (<em>Rhizobium meliloti</em> Dang.) from Pakistan and Nepal were studied in vials filled with sterile soil maintained at -0.03, -1.0, and -1.5 MPa. The main effects of water level, rhizobial strains and length of exposure to desiccation and their interactions showed a highly significant (P S 0.001) effect on the number of rhizobia g<sup>-1</sup> of soil. Growth and survival of rhizobia were highest in soil at -0.03 MPa followed by soil at -1.0 and -1.5 MPa. Highest cell counts were observed for strain UL 136 followed by strain UL 222 and the lowest was for strain UL 61. Two rhizobial strains (UL 136 and UL 222) were most tolerant to desiccation and showed highest growth and survival under low water potential. These two strains probably could be used as inoculants for alfalfa production under arid and semiarid environments.

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