Abstract
1. The amount of total nitrogen in the parts above ground and in the roots of papilionaceous plants (beans and lucerne) shows a marked increase following inoculation with specific and nonspecific nodule-farming bacteria and withPs. fluorescens. In the presence ofB. mycoides andA. chroococcum, the total nitrogen concentration in these plants increase only slightly as compared with the controls. 2. The free amino acid content of the parts above ground, and of the roots of beans and lucerne is increased by inoculating the plants with any of the test bacteria. The greatest increase is observed following the application of specific root nodule bacteria and ofPs.fluorescens. 3. The qualitative composition of the free amino acids in beans and lucerne treated with the test bacteria also changes, as compared with the controls, in relation to the species of bacteria. 4. The total nitrogen and free amino acid concentration in bean and lucerne plants with nodules grown under field conditions is several t mes higher than in plants without nodules.
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