Abstract

Using the soil of abandoned lead-zinc tailings and heavy metal enrichment plants (Lolium) as materials, through the outdoor pot experiment, study the influence of sludge on antioxidant enzymes (CAT, SOD and POD) activity and malondialdehyde (MDA) content. The results showed that: CAT activity increased with the increase of sludge concentration, increased first and then decreased, at concentration (278g/kg) to reach the highest: aerial parts 4.224 [u/(gFW.min)], roots1.532 [u/(gFW.min)]; SOD and POD activity increased with increasing sludge concentration trends, high concentrations (417g/kg) reached the highest, concentration of aerial parts and root SOD were 0.2134 [W/(h.gFW)] and 0.1376 [W/(h.gFW)]. Aerial parts of Lolium MDA content increased with sludge concentration increased, while roots was manifested in the opposite trend.The study showed that high concentrations (417g/kg) sludge can significantly improve antioxidant enzyme activities of Lolium, reduce cell damage and increase their resistance to lead-zinc stress.

Highlights

  • With China's rapid economic growth, environmental problems caused by the mineral mining process is an increasing number, especially the heavy metal pollution of soil

  • The results showed that: CAT activity increased with the increase of sludge concentration, increased first and decreased, at concentration (278g/kg) to reach the highest: aerial parts 4.224 [u/(gFW.min)], roots1.532 [u/(gFW.min)]; superoxide dismutase (SOD) and POD activity increased with increasing sludge concentration trends, high concentrations (417g/kg) reached the highest, concentration of aerial parts and root SOD were 0.2134 [W/(h.gFW)] and 0.1376 [W/(h.gFW)]

  • The CAT activity of Lolium aerial parts and roots growing in the lead-zinc tailings contaminated soils all increased under the sludge level of treatment, compared with the control (Figure 1)

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Summary

Introduction

With China's rapid economic growth, environmental problems caused by the mineral mining process is an increasing number, especially the heavy metal pollution of soil. Among them, adding paper sludge, urban sludge, urban life waste and other organic amendments can improve the matrix toxicity and increase nutrition and promote plant growth (Bao et al, 2008; Chen et al, 2008). These can promote the surface of plant growth at the same time, avoid the food chain, the potential threat to human life, so it is a good way to use to deal with the sludge and restore the ecological environment

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