The Influence of Singlet Oxygen Inhalations on Rats with Experimental Burn Trauma

Abstract

This article aims to study the dynamics of the crystallogenic properties of rat blood serum during a course of singlet oxygen inhalation in the post-burn period. The experiment was performed on 30 mature Wistar rats randomly divided into three groups of equal numbers. The first group (n=10) was intact, no manipulations were performed on the animals included in it, but only a single blood irradiation from the sublingual vein was performed. The animals of the second (control) and third (main) groups under combined anesthesia were subjected to thermal trauma according to our methodology, standard local treatment was carried out. Starting from the day following the injury, the rats of the main group were inhaled daily for 10 days of the airflow coming from the singlet oxygen generator. Blood samples from rats of all groups were collected and tested for crystallogenic properties and crystallogenesis-inducing activity. It was found that the inhalation of singlet oxygen in animals with severe thermal trauma contributes to the partial normalization of the crystallogenic activity of blood serum, which positively characterizes its rehabilitation potential. This trend must take place when assessing both the intrinsic crystallogenic and initiating properties of biological fluid, which is manifested both in the dynamics of morphological and morphometric indicators and the optical characteristics of serum facies.