Abstract
Post-traumatic muscle ischemia results from severe injury and can lead to muscle dysfunction. Therefore, patient management and treatment are very significant in all periods of injury. New methods are performed, especially using regenerative technologies to avoid complications and improve long-term outcomes. Objective. To determine histological changes in the muscles of the injured limb after traumatic ischemia after injection of platelet-rich plasma,Bone marrow stem cell concentrate (BMAC), and Stromal vascular fraction (SVF) prepared from adipose tissue on the 5, 15, and 30 days. Material and methods. Experiments were conducted on rabbits (Chinchilla breed). A tourniquet imposed on a lower limb, from the middle third of the thigh to the ankle joint. After 6 hours, the tourniquet was removed. The animals were divided into four groups: control, platelet-rich plasma, bone marrow stem cell concentrate, and stromal vascular fraction prepared from adipose tissue — histological muscle changes provided by Tescan Mira 3 LMU (Czech Republic) in scanning transmission electron microscopy. Results. On the 5th day after the experiment were no significant histological changes in muscles but in the contrary on the 15 days after experiment in BMAC and SVF groups detected new muscle fibers formation in necrotic areas and myonucleus organization. On the 30th day new angiogenesis was detected around muscle fibers. Platelet-rich plasma group characterized by massive connective tissue formation in necrotic areas. Conclusions. Necrosis and progressive muscle hypotrophy are unavoidably for this type of injury. It was shown that BMAC and SVF could stimulate regeneration and angiogenesis.
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