Abstract
Background.Metabolic reprogramming of tumor cells is one of the leading links in carcinogenesis. The objective to confirm the hypothesis of a propionate pathway for the synthesis of long-chain acids with an odd number of carbon atoms in cervical cancer.Materials and methods.As samples for the study were biopsies of the colli uteri, from which a suspension of tumor cells was obtained (20 – cervical cancer, 20 – cervical intraepithelial neoplasia III grade, 18 – endocervical scraping from healthy women). The spectrum of fatty acids (FA) was analyzed before, after 24 hours incubation without and with 50 μmol/l propionic acid by the gas chromatography method.Results.Metabolism of FA is multidirectional in different locus in cervical cancer. In the locus of cervical cancer, most likely, there is a propionic pathway for the synthesis of FA using palmitate. In cervical intraepithelial neoplasia III grade, the metabolism of FA is another. Palmitic acid was used for synthesis stearate, which was metabolized to oleic acid.Сonclusion.The specificity of biochemical changes within one organ and one pathology has been revealed, which reflects the stage-by-stage development of the oncological process.
Highlights
Metabolic reprogramming of tumor cells is one of the leading links in carcinogenesis
The objective to confirm the hypothesis of a propionate pathway for the synthesis of long-chain acids with an odd number of carbon atoms in cervical cancer
Metabolism of fatty acids (FA) is multidirectional in different locus in cervical cancer
Summary
Metabolic reprogramming of tumor cells is one of the leading links in carcinogenesis. P. The influence of propionate on the spectrum of long-chain fatty acids in cervical cells during dis- and neoplastic transformation. Цель исследования – оценить влияние пропионата in vitro на спектр ВЖК тканевых липидов эпителия шейки матки при предопухолевых заболеваниях и нео плазии экзоцервикса. Результаты исследования оценивали до и после 24-часовой инкубации с 50 мкмоль/л пропионовой кислоты и без нее (так называемая чистая, или изолированная, инкубация). Результаты В здоровых клетках цервикального эпителия после инкубации с пропионатом суммарное содержание насыщенных жирных кислот не изменилось, но среди отдельных представителей жирных кислот этого класса наблюдались количественные колебания После инкубации с пропионовой кислотой во всех биоптатах I группы независимо от локализации по отношению к очагу поражения наблюдалось увеличение концентраций С14:0, С15:0, С17:0, С18:0, С20:0 в среднем на 9,5 % для каждой из них в подгруппах IА и IБ по сравнению с соответствующим показателем в условиях.
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