Abstract
We investigated the effect of red light and plant growth regulators on somatic embryogenesis in China Rose (Rosa chinensis Jacq.). Embryogenic calli that had been induced by combinations of 2,4-dichlorophenoxyacetic acid and thidiazuron in darkness were exposed to dark, red, and white light treatments. Cultures subjected to red light treatment generated the greatest number of embryos, with one (SE1 embryos) or two (SE2 embryos) expanded cotyledons. The largest numbers of shoot-like embryos without cotyledons (SE0 embryos) were produced in cultures subjected to dark treatment. The effects of different concentrations of abscisic acid (ABA) on the proliferation and germination of different types of somatic embryos were also evaluated. A concentration of 9.45 μM was found to be the most effective in promoting the proliferation and germination of SE2 embryos. The higher the concentration of ABA (from 0 to 18.90 μM), the higher the percentage of abnormal polycotyledonary embryos produced. The highest percentage of regenerated plants was obtained from SE2 embryos.
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