Abstract
To study the influence of photoperiod on roots differentiation in the Tunisian grapevine (Vitis vinifera L.) cultivar Perle noir, roots and callus initiation were analyzed under three different conditions of day length: long day (LD), short day (SD) and darkness (D). The photoperiod influenced the number of callus and roots per cuttings; it has a significant effect on the roots and callus initiation. Expression profile analysis of six MADS-box genes (VTM8, VSEP2, VAG12, VAG17-1, VAG17-2 and VSOC1.3) during root and callus development is in agreement with the above-mentioned observation. The expression of the MADS-box genes during root and callus development fluctuated in a tissue-dependent manner. These data suggest that all genes are expressed in roots under three photoperiods. Total darkness gives the number of the most important root per cutting compared to the other two conditions. This photoperiodic condition gave the most important expression of the studied genes VAG12, VAG17-2, VAG17-1, VTM8 and VSEP2 transcripts were not found in callus grown in the dark or in LD conditions, respectively. VSOC1.3 transcripts were not found in callus grown in the dark or in SD conditions, respectively. Transcript abundance of VTM8 and VSOC1 was highest in LD.
Highlights
Certain shoot and root cells can be activated to produce a new one, each with a new meristem at its tip
The two statistical analysis including and excluding the cuttings without roots showed that there were significant differences between cuttings grown under D and long day (LD) conditions and between D and short day (SD) conditions respectively (Table 2) but there were no significant differences between cuttings grown under SD and LD conditions
The highest number of roots per cuttings for Perle noir cultivar was obtained in the total darkness
Summary
Certain shoot and root cells can be activated to produce a new one, each with a new meristem at its tip. (2015) The Influence of Photoperiod on the Regulation of Root and Callus Initiation of Perle Noir (V. vinifera L.): Expression of MADS-Box Gene. The formation of the lateral roots in the root system provides a good model for studying how plant development is coordinated with environment. Some studies suggest that specific pericycle cell exists This suggests that environmental conditions influence lateral root formation [6]. Favre (1973) [12] was the first to study the histological detail of adventitious root formation in Vitis canes, and identify the initial step as the appearance of “swelling” or hypertropic nuclei within clusters of cells of the interfascicular cambium. Plants use light as a source of information to optimally adapt growth and development to the ambient environment. We were further interested in understanding of the genetic and molecular control of root initiation in the grapevine
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
