The influence of pancreatic and duodenal extracts on the glycosuria and the respiratory metabolism of depancreatized dogs

Abstract

Several dogs completely depancreatized by Hedon's method and eliminating glucose and nitrogen in Minkowski's ratio were treated by intravenous injection of pancreatic extract prepared by Knowlton and Starling's method.1 The urines collected in twenty-four hour periods exhibited an increase in the D: N ratio on the days immediately following. When the urine was collected in short periods after injection, a marked fall in the sugar output was witnessed lasting from four to ten hours but this was followed by a compensating rise which, in some instances, augmented the total for the twenty-four hour period, in others raised it only to the previous level.Much greater effects were obtained with a double extract of dog's pancreas and duodenal mucosa. The following experiment (No. II) is typical. The effect, however, cannot be ascribed to the organic extract from either the pancreas or the duodenum for the same Ringer's solution in which the tissues were extracted when made alkaline to about the same extent with N...