Abstract
Microbial biomass nitrogen and extractable organic nitrogen in extractions by 0.05M K<sub>2</sub>SO<sub>4</sub>&nbsp;and 0.01M CaCl<sub>2</sub>&nbsp;were studied in a&nbsp;long-term experiment with successive growing of silage maize. The highest content of microbial biomass nitrogen was measured for manure treatment, by 38&ndash;133% higher than for the control. In treatments with applications of mineral nitrogen fertilizers microbial biomass N was lower on average by 22&ndash;30% against the control. Extractable organic nitrogen was also lower in treatments with mineral N fertilizers compared to the control: by 23% in ammonium sulphate treatment and by 29% in DAM. The highest content of extractable organic nitrogen was determined for manure treatment. There was a&nbsp;positive correlation (r = 0.44&ndash;0.9) between microbial biomass nitrogen and extractable organic nitrogen in the extractions by 0.01M CaCl<sub>2</sub>&nbsp;and 0.05M K<sub>2</sub>SO<sub>4</sub>.
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