The influence of octreotide to the activity of hepatic stellate cells and in the process of liver fibrosis authors

Abstract

Objective To investigate the influence of octreotide to the activity of LX-2,a hepatic stellate cell line,at different concentration,and explore its effect and mechanism in the process of liver fibrosis. Methods Methyl thiazol tetrazolium(MTT)assay was conducted for the influence of octreotide to the proliferation of LX- 2. The mRNA levels of transforming growth factor-β1 (TGF-β1),α- smooth muscle actin(α- SMA), smad- 4 and c- Jun under octreotide were detected by reverse transcriptase- polymerase chain reaction(RT- PCR).The content of type-Ⅰcollagen in the supernant of cultured LX- 2 was assayed by enzyme linked immunosorbent assay (ELISA).Whether c- Jun regulates TGF-βthrough binding to its promoter was monitored based on small interfering RNA(siRNA)and chromatin immunoprecipitation(ChIP)technologies. Results The proliferation of LX-2 was inhibited obviously by octreotide in a concentration- dependent manner, the inhibition rates of which were 27.5% and 49.8% at 10-7 nmol/L and 10-5 nmol/L. Compared with control, the transcriptions of TGF-β1,α- SMA, smad- 4 and c- Jun were down - regulated significantly under octreotide(P<0.05), and the content of type-Ⅰcollagen reduced significantly(P< 0.05).The expression of TGF-β1 reduced significantly under interference of c-Jun. Conclusion Octreotide could inhibit the proliferation of LX- 2, reduce the expressions of TGF -β1,α- SMA,Smad- 4 and c- Jun, regulate TGF-β1,and reduce the secretion of type-Ⅰcollagen, and retard the occurrence of fibrosis, provided important clinical significance to control portal hypertension. Key words: Octreotide; Hepatic stellate cell; Liver fibrosis