The Influence of Nystatin and Argon Laser Combination on Candida Albicans and Actinomycetes Isolated from Oral Cavity of Children

Abstract

The present study has been undertaken to illustrate the effect of argon laser and nystatin on isolated candida albican andActinomycetes from oral cavity of children. Out of 20 samples, positive cultures were 26 bacterial samples and 12 fungussamples, the isolated bacteria included Staphylococcus aureus ,klebseilla pneumonias ,and streptococcus viridans .The isolated fungi included candida albican and Actinomycetes. The present study shows that determination of minimal inhibitoryconcentrations of nystatin ranged from 1-12 i.u./ml in sabroud agar plates were prepared and cultured with tested fungi ,incubatedat 25 oC for 24-48 hrs.The candida albican and Actinomycetes fungi exposed to argon ion (Ar+) laser after focal with a lens of(15) cm after focal point for periods of time(1,1.5,2,4,6,8,10,12,14,16)min, and shows the effect of combination between nystatin and laser light.The result showed that the minimal inhibitory concentrations of nystatin for candida albican were 1-12 i.u./ml but the minimal inhibitory concentrations of nystatin for Actinomycetes were 3-12 i.u./ml .Also it is found that candida albican completely killed after exposure to argon laser for 12-16 minutes. While Actinomycetes completely killed after exposure to argon laser for 10-16 minutes.The combination between laser and nystatin were more effective,all candida albican isolated from oral cavity were killedafter exposure to (argon laser light , nystatin combination) for 10 minutes / 7 i.u./ml, while all Actinomycetes killed after exposureto (argon laser light ,nystatin combination) for 6 minutes/5 i.u./ml .The widly uses of laser in oral and dental treatment encourages to further investigations . Recent advances has shown the save and destructive results by using laser open the hopes to introduce laserstrongly in treatment of oral and dental diseases