Abstract
The usual method of tracer analysis for calculating the flow across a biological membrance is based on the assumption that the compartments on either side are well-stirred. Thus, the validity of the rate of flow determination is questionable for cases where the distribution of tracer is not homogeneous. In this study, a mathematical model is developed for the purpose of estimating the effect of slow mixing on the calculation of the flow rate. The model is applied to the measurement of the rate of flow of aqueous humor through the living eye by use of a fluorescent dye as a tracer. A transit time of several minutes for the passage of fluorescein through the posterior chamber and an extended period of nonuniform distribution of fluorescein in the anterior chamber was observed. The effect of slow mixing on the calculated flow rate is compared to rates derived from equations based on the assumption of rapid mixing. Aqueous flow rates determined by the two methods were found to agree to within ≈20%.
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