The influence of N2-fixation on the carbon balance of leguminous plants

Abstract

Biological dinitrogen fixation by legume-rhizobia symbiosis is very important both from the economic and from the ecological point of view. Theoretically, the reduction of the N2-molecule to ammonia requires at least 16 ATP and 1.5 mg C per mg N fixed (Nfix). These values are difficult to determine in situ as this necessitates the determination of that part of root respiration which drives N2-fixation. New approaches to such determinations and the results obtained are described. The values vary, depending on the plant species studied, the developmental stage of the plants and the genetic variability of macro- (and micro-?) symbionts. The values range between 1.5 and 4 mg C/mg Nfix. In some species (e.g.Vicia faba L. cv. Fribo), the apparent CO2 assimilation is enhanced in order to meet this high energy need. In others (e.g.Pisum sativum L. cv. Grapis), root growth is restricted. Physiological criteria are discussed which allow an early diagnosis of the energetic efficiency of various combinations of macro-and microsymbionts as a basis for a selection in plant breeding.