Abstract
Symbiotic arbuscular mycorrhizal fungi (AMF) play a major role in plant development, growth, and relationships with the environment through a change in the accumulation of secondary metabolites; hence, we planned to investigate AMF’s influence on sennoside A and B accumulation in Senna alexandrina (SA) and Senna italica (SI). Seeds of SA (S. alexandrina free of mycorrhizae) and SI (S. italica free of mycorrhizae) were planted in two types of soils: +mycorrhiza and—mycorrhiza. The plant leaves of SA, SI, S. alexandrina with mycorrhizae (SAM) and S. italica with mycorrhizae (SIM) were collected and extracted (with 85% methanol), and sennoside A and B content was evaluated by the HPLC–UV method. The antioxidant activity of SA, SI, SAM and SIM was evaluated by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, while antimicrobial properties were evaluated by the minimum inhibitory concentration method (MIC). The AMF colonization was 85.66% and 85%, respectively, in the roots of SA and SI. The HPLC analysis showed a significant increase in (%) the content of sennoside A/sennoside B by 71.11/88.21, respectively, in SAM and 6.76/36.37 in SIM, which clearly indicated positive AMF effects. The DPPH/ABTS [The half maximal inhibitory concentration (IC50): 235.9/321.5 µg/mL] scavenging activity of SAM was comparatively higher and it also exhibited strong antibacterial action (MIC: 156.25 µg/mL), which supported the increase in sennoside content. This finding may be useful for further investigations of the symbiotic relation of mycorrhizal fungi with other plant species.
Highlights
Leguminosae is one of the most widespread plant families in the world, containing the genus Senna, which includes hundreds of plant species [1]
Italica was 85.6 and 85%, respectively. This ratio indicated the strength of the relationship between mycorrhizae and plants, and the dependence of each was determined by the growth of both plants after 5 months of cultivation in growth chambers with continuous monitoring (Figure 2A)
We investigated the effect of mycorrhizal fungi present in soil on the accumulation of the secondary metabolites sennosides A and B in Senna alexandrina and Senna italica using the High-Performance Liquid Chromatographic (HPLC) method, and determined their antioxidant and antimicrobial potential
Summary
Leguminosae is one of the most widespread plant families in the world, containing the genus Senna, which includes hundreds of plant species [1]. Senna alexandrina and Senna italica contain safe medical compounds (sennoside A and sennoside B) that are used to treat many diseases, especially constipation problems [2,3]. Increases bowel movements and reduces water absorption, which imparts their. Mycorrhizal fungi are one of the most important soil microorganisms and major components of a sustainable soil–plant system. It is in the second-largest components of the soil [7] where these fungi establish a symbiotic relationship with more than 80%
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
