The influence of mycorrhizal colonization on growth in the greenhouse and on catechin, epicatechin and procyanidin in roots of Fagus sylvatica L.

Abstract

The influence of mycorrhizal colonization on beech (Fagus sylvatica L.) root tannin (procyanidin polymer) and its putative precursors catechin and epicatechin was investigated by high performance liquid chromatography. Seedlings planted in a sterile mixture of litter, compost, soil and sand were inoculated with brown beech ectomycorrhizas collected from a woodland (Lactarius subdulcis Bull ex Fr. × F. sylvatica). The seedlings were not fertilized during the first year of growth. Nonmycorrhizal control plants showed severe nutrient-deficiency symptoms on their leaves and grew less well than mycorrhizal plants. Mycorrhizal roots contained significantly less catechin, epicatechin and procyanidin polymer than nonmycorrhizal roots. In the second year of growth, the plants were fertilized and procyanidin formation in roots was investigated. None of the fertilized plants showed mineral-deficiency symptoms. Fertilized mycorrhizal roots consistently contained significantly less catechin and epicatechin than nonmycorrhizal controls, but procyanidin polymer content varied between replicate experiments. The possible function of catechin and epicatechin in ectomycorrhizal formation is discussed.