The influence of monochromatic lights on flavonoid production by the fungus Sanghuangporus vaninii: Modeling of kinetic profiles and expression levels of important genes in flavonoid synthesis

Abstract

• The kinetic model of the effect of light on flavonoids of fungi was studied. • Kinetic models were combined with the expression levels of related enzymes. • The yield of flavonoids increased by 25.2 % under blue light. • The content of was the highest under blue light. • The expression levels of pal , 4 cl , f3 ' 5 ' h were up-regulated under blue light. Four different monochromatic lights (blue, red, yellow and green) were used to study their separate influence on the growth, morphology and flavonoid accumulation by S. vaninii compared to under white. The results showed that the accumulation rate of total flavonoids under blue light was faster, while the total flavonoid accumulation rate under red light was slower than under white light, and other monochromatic lights had little effect on the synthesis of total flavonoids. The kinetic model parameters and fitting values are basically consistent with the experimental values. Through the development of kinetic model and model validation, the maximum flavonoid concentration under blue light increased by 25.2 % compared with that under white. The relative expression of phenylalanine ammonia lyase (PAL), 4-coumaric acid coenzyme A ligase (4CL) and flavonoid-3′, 5′-hydroxylase (F3′, 5′H) genes were significantly up-regulated under blue light. The influence of lights on the fungal flavonoid production was in part in line with the key genes expression involved.