Abstract
The purpose of this work to determine the influence of different concentration of methotrexate on apoptosis level in the ovaries of Drosophila melanogaster at normal and elevated temperature during development. In the experiment was used 300 hybrid individuals “Oregon-R x vg” with spontaneous mutation like Beadex phenotype. We investigated six group: control, a group after the methotrexate 400 mkg/ kg medium treatment, a group with 2000 dose, a group with 10000 dose, temperature 28 C treatment and a group after methotrexate 400 and temperature 28 C treatment together. During the experiment we found that apoptosis in checkpoint #1 reaches to maximum values of the group MTX 10000 and of group after the temperature 28 C treatment. There was also a dose dependence on the intensity of apoptosis from increased concentration in checkpoint #1. The experimental group MTX 400 has the maximum intensification of cellular ath in the checkpoint #2, which is not observed in the other groups. Due to the fact that the 400mkg/kg is close to 50LD, probably most of the individuals survive. The high index of the presence of apoptosis observed in control group of this hybrid strain. Correlations between the increase in MTX dosesang degree of ovarian development were not observed. However, the 2000 MTX group has a high rate of atrophy of both ovaries.
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