The Influence of Malassez Epithelial Residue in Inflammatory Periodontal Tissues on the Number of Periodontal Stem Cells and Their Differentiation Ability

Abstract

To investigate the effect of Malassez epithelial surplus in inflammatory periodontal tissues on the number and differentiation ability of periodontal stem cells. 30 patients with periodontitis admitted to the hospital from November 2014 to April 2017 were selected as the observation group. A total of 30 healthy oral examination patients were selected as control group. Periodontal tissue in the 2 groups were taken and the periodontal ligament cells, the complete inflammatory periodontal ligament stem cells by using the low density screening, purification, and periodontal tissue were obtained by using differential digestion method of Malassez epithelial remaining, were observed under inverted microscope in the periodontal tissue Malassez epithelial residual form, will be in the periodontal tissue Malassez epithelial surplus together with inflammatory periodontal ligament stem cells, RT-PCR technique has been applied to the determination of ALP and Runx-2 mRNA expression level to observe the differentiation ability. Flow cytometry showed that the cell markers CD29 and CD90 in the cultured periodontal stem cells were positive (88.9% and 99.7%, respectively). A hematopoietic stem cell marker CD45 was negative (0.3%). The inverted microscope results showed that the residual epithelial cells of Malassez, isolated and cultured, had a large nuclear to cytoplasmic ratio, and the different cells were arranged relatively closely, with the overall cell structure of paving stone. The expression levels of ALP and runx-2 mRNA in the observation group were higher than those in the control group (P<0.05). Malassez epithelial surplus in inflammatory periodontal tissues can promote the proliferation of periodontal stem cells and improve the ability of osteogenic differentiation.