Abstract
AbstractVarious aspects of the reaction of Michaelis and Salomon (increased oxygen consumption of mammalian erythrocytes after the addition of liver‐extract) have been studied and compared with the methylene‐blue respiration of the erythrocytes.The most important facts are the following:1) Each of the individual components of the system could be made a limiting factor by increasing the concentration of the other components. In methylene‐blue respiration both components are limiting factors at all concentrations.2) Erythrocytes of man and the rabbit reacted with liver‐extract; those of hogs sometimes did. Rat erythrocytes did not react. An interesting correlations between this reactivity and the blood catalase level was demonstrated No difference was observed in the behaviour of erythrocytes from rabbits and rats with methylene‐blue as an activator.3) In contradiction with current literature, no abnormal reaction of erythrocytes of patients, suffering from pernicious anaemia, was found.4) The intactness of the cell proved to be in no way necessary, though other authors suppose that it is. Haemolysed erythrocytes, freed from cell‐remnants reacted like a suspension of erythrocytes, provided the haemoglobin concentration was of the same magnitude. Methylene‐blue does not increase the oxygen consumption of haemolysed erythrocytes.5) The active principle of the liver‐extract proved to be thermolabile and destroyable by irradiation with ultraviolet light.6) Antianaemic liver preparations were inactive.7) Oxygen consumption was increased in the presence of fluoride. This effect is probably caused by an inhibition of glycolysis and therefore suggests consumption of carbohydrates (Pasteur‐Meyerhof reaction).8) Carbon dioxide was produced during the reaction. The respiratory quotient was about 0.9.
Published Version
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