Abstract
This article reports the efficacy of percutaneous autologous leukocyte- and platelet-rich plasma (L-PRP) injection into delayed union site as a minimally invasive method alternative to bone marrow aspirate and open grafting techniques. Each of 15 participants was followed on a regular basis with clinical examinations, roentgenograms. The average time to union was 8.4 weeks after L-PRP injection and the union was achieved in all cases. CD34+/45+ cells counts were increased by 410% and CD34+/45- cells counts were increased by 488% on average in L-PRP. Our investigation showed that L-PRP enrich in stem cells can produce the desired stimulatory response despite a substantial amount of vital bone cells from mesenchymal line. We believe that the using of L-PRP enriched in stem cells, growth factors and antimicrobial proteins might be a promising treatment method in regenerative medicine.
Highlights
Due to their numerous key properties, platelets started to be used in the contemporary medicine in the strategies of stimulation of tissue healing, as an autologous cell and plasma fraction extracted from the peripheral blood [1]
The autologous whole blood undergoes centrifugation in order to obtain a concentrate of platelets—called platelet-rich plasma (PRP) like in transfusion medicine—which could be transformed into a fibrin gel after activation by the addition of thrombin and calcium ions
In Aspenberg and Virchenko studies leukocyte- and platelet-rich plasma (L-PRP) was irradiated at 25 Gy to inactivate the WBCs, and truly platelet concentrate was reached [25]
Summary
Due to their numerous key properties, platelets started to be used in the contemporary medicine in the strategies of stimulation of tissue healing, as an autologous cell and plasma fraction extracted from the peripheral blood [1]. The autologous whole blood undergoes centrifugation in order to obtain a concentrate of platelets—called platelet-rich plasma (PRP) like in transfusion medicine—which could be transformed into a fibrin gel after activation by the addition of thrombin and calcium ions (or equivalent technologies). The impact of platelet growth factors was intensively investigated and debated in trauma and maxillofacial applications [3]-[13], and PRP/PRF technology for regenerative medicine treatments is one of the most active fields of research in the dental/maxillofacial and orthopedic literature, with implant surfaces/designs and bone bioengineering [14]-[20]. We report the influence of L-PRP on regeneration of tibial fractures. To our knowledge, this is the first report of concentrated stem cells in L-PRP harvested from blood
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