Abstract
Intact tobacco leaves sprayed daily with kinetin (0·1 mg 1 −1) begining 9 days prior to inoculation with tobacco ringspot virus (TRSV) exhibited high levels of virus infectivity whereas leaves sprayed as above with higher levels of kinetin (1·0 mg 1 −1 and 10·0 mg 1 −1) exhibited appreciably lower levels of virus than the control plants. Conversely, the extent of viral infectivity in tobacco leaves, sprayed daily with kinetin at the three different concentrations (0·1, 1·0 and 10·0 mg 1 −1) beginning 3 days prior to inoculation, showed no appreciable difference in the pattern of virus infectivity from that of non-sprayed inoculated leaves. Extracts of infected and non-infected tobacco leaves were assayed for cytokinin activity by the radish cotyledon expansion test. Leaf extracts of TRSV-infected plants contained less cytokinin activity than leaf extracts from non-infected plants.
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